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KMID : 0545120000100060836
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 6 p.836 ~ p.839
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Beef Tallow Hydrolysis by Immobilized Lipase
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KIM, DONG-JOON
SHIN, DONG-HOON/HUR, BYUNG-KI/HUR, BYUNG-KI
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Abstract
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Beef tallow, which is an industrial lipid substrate, was hydrolyzed by lipase immobilized on a high-density polyethylene (HDPE) powder. Ethanol pre-washing process affected the immobilization efficiency. Half-life of storage of the HDPE at 4℃ was 150days. And after 10times of repeated use, more than 50% of initial activity remained. An apparent Michaelis constant (K_m) and maximum velocity (V_max) were 2.7M, and 1.4m㏖/min/l for immobilized lipase, and 0.5M, and 1.9m㏖/min/l for soluble lipase, respectively.
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